stratascript h-reverse transcriptase Search Results


trizol solution  (Thermo Fisher)
99
Thermo Fisher trizol solution
Trizol Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/trizol solution/product/Thermo Fisher
Average 99 stars, based on 1 article reviews
trizol solution - by Bioz Stars, 2026-06
99/100 stars
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rneasy total rna kit  (Qiagen)
99
Qiagen rneasy total rna kit
FIGURE 1. Der f dose dependently induces the expression of proin- flammatory mediators of AMs. AMs were stimulated for 24 h with a non- cytotoxic dose of Der f, OVA, or LPS. The accumulation of proinflam- matory mediators in supernatants was evaluated (see Materials and Methods). Data are the means SEM of three to six separate experiments performed in triplicate. , p 0.05 compared with medium control (A). <t>Total</t> <t>RNA</t> extracted from stimulated AMs was evaluated by RT-PCR. A representative RT-PCR profile from three independent experiments is shown (B). Nuclear proteins were extracted from AMs 24 h after incuba- tion with Der f, and activation of NF-B was evaluated by EMSA. Super- shift was performed using Abs to the p50 (p50) and p65 (p65) subunits of NF-B. Unlabeled oligonucleotides containing binding sites for NF-B (B), AP-1, and specificity protein 1 were used for competition. Figure is representative of three independent experiments (C).
Rneasy Total Rna Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rneasy total rna kit/product/Qiagen
Average 99 stars, based on 1 article reviews
rneasy total rna kit - by Bioz Stars, 2026-06
99/100 stars
  Buy from Supplier

Image Search Results


FIGURE 1. Der f dose dependently induces the expression of proin- flammatory mediators of AMs. AMs were stimulated for 24 h with a non- cytotoxic dose of Der f, OVA, or LPS. The accumulation of proinflam- matory mediators in supernatants was evaluated (see Materials and Methods). Data are the means SEM of three to six separate experiments performed in triplicate. , p 0.05 compared with medium control (A). Total RNA extracted from stimulated AMs was evaluated by RT-PCR. A representative RT-PCR profile from three independent experiments is shown (B). Nuclear proteins were extracted from AMs 24 h after incuba- tion with Der f, and activation of NF-B was evaluated by EMSA. Super- shift was performed using Abs to the p50 (p50) and p65 (p65) subunits of NF-B. Unlabeled oligonucleotides containing binding sites for NF-B (B), AP-1, and specificity protein 1 were used for competition. Figure is representative of three independent experiments (C).

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: House dust mite Dermatophagoides farinae augments proinflammatory mediator productions and accessory function of alveolar macrophages: implications for allergic sensitization and inflammation.

doi: 10.4049/jimmunol.170.1.528

Figure Lengend Snippet: FIGURE 1. Der f dose dependently induces the expression of proin- flammatory mediators of AMs. AMs were stimulated for 24 h with a non- cytotoxic dose of Der f, OVA, or LPS. The accumulation of proinflam- matory mediators in supernatants was evaluated (see Materials and Methods). Data are the means SEM of three to six separate experiments performed in triplicate. , p 0.05 compared with medium control (A). Total RNA extracted from stimulated AMs was evaluated by RT-PCR. A representative RT-PCR profile from three independent experiments is shown (B). Nuclear proteins were extracted from AMs 24 h after incuba- tion with Der f, and activation of NF-B was evaluated by EMSA. Super- shift was performed using Abs to the p50 (p50) and p65 (p65) subunits of NF-B. Unlabeled oligonucleotides containing binding sites for NF-B (B), AP-1, and specificity protein 1 were used for competition. Figure is representative of three independent experiments (C).

Article Snippet: Total cellular RNA was extracted from pooled AM samples (RNeasy Total RNA kit; Qiagen, Hilden, Germany) and converted to cDNA with StrataScript H-reverse transcriptase (Stratagene, La Jolla, CA).

Techniques: Expressing, Control, Reverse Transcription Polymerase Chain Reaction, Activation Assay, Binding Assay